Image Analysis or Stereology

Image Analysis or Stereology: Which to Choose for Quantifying Fibrosis?

Bias in image analysis and its solution: unbiased stereology

Although the human eye is excellent for pattern recognition, it often lacks the sensitivity to detect subtle changes in particle density. Because of this, quantitative evaluation may be required in some studies. A common type of quantitative assessment used for routine toxicology studies is two-dimensional histomorphometry. Although this technique can provide additional information about the ti...

Stereology.

1.1 組織標本を使用する定量解析が抱える諸問題 組織標本の解析は定性的な所見から始まる.顕微鏡を用いた丁寧な観察によって初めて得られる検体の 大きさや分布,形態などについての詳細な情報は,全ての研究の基礎となるべき重要なものである.しか しながら,定性的なデータだけでは,統計学的な検討が難しく,結果が主観的なものになりやすい.この ため,生理的組織と病的組織の比較など,さらなる研究を行う際には,定量的なデータが必要になる.定 量解析の基本的な命題は,いかにして検体の正確な数を客観的に求めることができるか,ということであ る.組織標本に基づく定量解析は単純に思われるが,正確なデータを得ることは実は容易ではない. 最大の問題は,サンプリングバイアスである(図1).これは,標本抽出の際に母集団を代表していない 検体が選ばれるために生じる偏りのことであり,2次元的な切片を使って定量解析を行...

Computer-assisted stereology and automated image analysis for quantification of tumor infiltrating lymphocytes in colon cancer

BACKGROUND Precise prognostic and predictive variables allowing improved post-operative treatment stratification are missing in patients treated for stage II colon cancer (CC). Investigation of tumor infiltrating lymphocytes (TILs) may be rewarding, but the lack of a standardized analytic technique is a major concern. Manual stereological counting is considered the gold standard, but digital pa...

Quantification of myocardial fibrosis by digital image analysis and interactive stereology

BACKGROUND Cardiac fibrosis disrupts the normal myocardial structure and has a direct impact on heart function and survival. Despite already available digital methods, the pathologist's visual score is still widely considered as ground truth and used as a primary method in histomorphometric evaluations. The aim of this study was to compare the accuracy of digital image analysis tools and the pa...